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. 2021 Jul 29;10(8):1226. doi: 10.3390/antiox10081226

Figure 4.

Figure 4

Effect of Garcinia cambogia on NRF2-ARE activation in FFA-induced HepG2 cells. (A) Effect of G. cambogia (20–80 μg/mL) on NRF2 expression in FFA-treated HepG2 cells (n = 4 per group). (B) Effect of G. cambogia on NRF2 nuclear expression in FFA-treated HepG2 cells. Cells were treated with G. cambogia (40 and 80 μg/mL) for 12 h, and cells were fractionated into nucleus compartment as described in the Methods section (n = 4 per group). (C) Representative immunofluorescence images of NRF2 in FFA-treated HepG2 cells. Colocalized FITC (i.e., NRF2) and DAPI (i.e., nuclei) were quantified using ImageJ software, and relative intensities in the nuclear and cytoplasmic fractions were expressed as a histogram. Scale bars: 20 μm (n = 5 per group). (D) Effect of G. cambogia on ARE promoter activity in FFA-treated HepG2 cells. ARE-luc vector-transfected HepG2 cells were cotreated FFA and G. cambogia (20–80 μg/mL) for 12 h and detected ARE promoter activity (n = 6 per group). (E) Effect of G. cambogia (20–80 μg/mL) on the transcript levels of HMOX1 and SOD1 in FFA-treated HepG2 cells (n = 5 per group). ** p < 0.01 vs. Con, # p < 0.05 and ## p < 0.01 vs. FFA. Data are mean ± S.D.