Table 3.
Secondary metabolites quantified by UHPLC coupled to Qtrap 6500 (AbSciex).
| EB | ET | EM | AB | AM | Sign. | |
|---|---|---|---|---|---|---|
| Anthocyanins | ||||||
| 11 | 2685.00 ± 42.43 | nd | 548.87 ± 41.43 | 4467.50 ± 236.88 | 1975.90 ± 107.38 | ** |
| 12 | 2671.05 ± 43.78 | nd | 77.68 ± 2.77 | 3766.25 ± 290.06 | 88.04 ± 7.50 | ** |
| 17 | 651.56 ± 22.09 | nd | 158.47 ± 31.40 | 948.44 ± 71.82 | 499.30 ± 115.04 | ** |
| 21 | 54.84 ± 3.09 | nd | nd | 62.54 ± 0.16 | nd | ** |
| 30 | 1780.00 ± 120.21 | nd | 383.25 ± 0.35 | 3762.50 ± 434.87 | 2907.50 ± 152.03 | |
| Flavanols | ||||||
| 14 | nd | 6.47 ± 0.01 | nd | nd | nd | ** |
| 19 | nd | 23.60 ± 3.39 | nd | nd | nd | ** |
| 22 | nd | 5.06 ± 0.51 | nd | nd | nd | ** |
| Flavonols | ||||||
| 26 | 232.70 ± 1.15 | 0.91 ± 0.02 | 211.45 ± 0.47 | 265.44 ± 16.25 | 222.22 ± 2.74 | ** |
| 32 | 451.24 ± 21.34 | 1.68 ± 0.60 | 260.48 ± 5.09 | 720.20 ± 1.64 | 424.15 ± 10.95 | ** |
| 33 | 1842.50 ± 222.74 | 27.59 ± 21.2 | 915.00 ± 141.42 | 3442.50 ± 1686.45 | 1667.50 ± 258.09 | ** |
| 36 | 1850.00 ± 45.96 | 7.36 ± 0.14 | 1835.00 ± 14.14 | 1837.50 ± 3.53 | 1872.50 ± 53.03 | ** |
| 53 | 240.75 ± 10.25 | 0.91 ± 0.01 | 209.25 ± 0.35 | 262.50 ± 4.24 | 221.25 ± 1.06 | ** |
| Other compounds | ||||||
| 6 | 205.00 ± 10.05 | nd | nd | 390.00 ± 14.14 | nd | ** |
| 47 | nd | 23.92 ± 9.40 | nd | nd | nd | |
| 40 | nd | 1.91 ± 0.97 | nd | nd | nd | |
EB = ethanolic extract of blackberry fruits; ET = ethanolic extract of almond skin; EM = ethanolic extract of blackberry jam; AB = anthocyanin extract of blackberry fruits; AM = anthocyanin extract of blackberry jam. Results are given as means ± standard deviation (SD). nd: not detected. Concentrations are reported as µg/g DW; anthocyanins [cyanidin 3-O-glucoside (11), pelargonidin 3-O-glucoside (12), cyanidin xyloside (17), cyanidin dioxayl glucoside (21), delphinidin 3-O-glucoside (30)], flavanols [(+)-catechin (14), proanthocyanidin B (19), and (-)-epicatechin (22)], and flavonols [quercetin 3-O-rhamnoside (26), quercetin 3-O-glucoside (32), kaempferol 3-O-glucoside (33), kaempferol 3-O-rutinoside (36), quercetin (53)], chlorogenic acid (6), rosmarinic acid (47), and prunin (40)]. Means within a row with different letters are significantly different by Tukey’s post hoc test. ** Significance at p < 0.01.