Table 2.
MIC and MBC assays of nanotitania against Klebsiella pneumoniae ATTC 4352.
| Test Tube | Dilution (Ratio) |
Sample Strength (mg/mL) | MIC | MBC | ||
|---|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | ||||
| 1 | 1:1 | 50 | T | N | N | N |
| 2 | 1:3 | 25 | T | G | G | G |
| 3 | 1:7 | 12.5 | T | G | G | G |
| 4 | 1:15 | 6.25 | T | G | G | G |
| 5 | Positive control | T | G | G | G | |
| 6 | Negative control | C | N | N | N | |
| Test inoculum size | 3.90 × 106 CFU/mL | |||||
| Test tube 5: Purity check | Pure strain | |||||
| Test tube 5: Viable count | 4.10 × 104 CFU/mL | |||||
| Test tube 6: Medium sterility | Clear and sterile | |||||
Notes: T (turbid broth test tube), C (clear broth test tube), N (no growth observed on agar medium), G (growth observed on agar medium), CFU (Colony forming unit).