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. 2021 Jul 26;10(8):1189. doi: 10.3390/antiox10081189

Figure 3.

Figure 3

Maclurin showed radical scavenging property and activated ARE signaling. (A,B) ABTS (A) and DPPH (B) radical scavenging activity of maclurin. The results are shown as the mean ± SD of three replicates. (C) HaCaT cells were firstly transfected with the ARE-Luc reporter and a Renilla-luciferase vector using the DharmaFECT® Duo transfection reagent. After incubation for 24 h, the cells were treated with maclurin for 14 h. The cells were lysed and subjected to the luciferase reporter assay. The data are shown as the mean ± SD of three replicates. * p < 0.05 vs. untreated control. (D,E) HaCaT cells were incubated with maclurin for the indicated time (1–8 h). The cells were harvested and subjected to Western blots and RT-PCR for HO-1 and Nrf2. The data are shown as the mean ± SD of three replicates. * p < 0.05 vs. untreated control. Vit C: vitamin C, Mac: maclurin.