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. 2021 Jul 21;10(8):1158. doi: 10.3390/antiox10081158

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of UCP pre-incubation on MGO-induced intracellular ROS production. (a) The representative photos of DCFH-DA green fluorescence probe detecting intracellular ROS in a single-cell configuration analyzed by imaging flow cytometer. (b) Flow cytometry histogram of relative intracellular ROS levels in each sample group. (c) A graphical representation of percent relative fluorescent intensity of cell populations in each treatment group. Data are presented as mean ± SEM of n ≥ 3. *, p <0.05; and ***, p < 0.001, when compared with the vehicle-treated group; †, p < 0.05; and †††, p < 0.001, when compared with MGO-treated group.