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. 2021 Jul 30;10(8):1232. doi: 10.3390/antiox10081232

Figure 3.

Figure 3

Increased DNMT3B binding and reduced H3K36me3 occupancy is associated with PITPNC1 and LINC00910 downregulation and hypermethylation of the enhancer regions in response to PTS in breast cancer cells. (A) PITPNC1 and LINC00910 expression upon 9-day treatment of MCF10CA1a breast cancer cells with 7 μM PTS as determined by QPCR. (B) Average methylation status of CpG sites within PITPNC1 and LINC00910 enhancer regions as determined by pyrosequencing in MCF10CA1a breast cancer cells treated for 9 days with 7 μM PTS. Gene maps show transcription start site (TSS) indicated by +1, transcription factors predicted using Transfac, and pyrosequenced CpG sites circled and numbered. The blue-shaded fragment in the gene map represents the DNMT3B-increased and H3K36me3-reduced overlapping peaks. The purple-shaded regions refer to a region tested in pyrosequencing. All results represent mean ± SD of three independent experiments; *** p < 0.001, ** p < 0.01, * p < 0.05.