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. 2021 Jul 30;10(8):1232. doi: 10.3390/antiox10081232

Figure 4.

Figure 4

Increased DNMT3B binding and reduced H3K36me3 occupancy is associated with PITPNC1 and LINC00910 downregulation and hypermethylation of the enhancer regions in response to PTS in breast cancer cells. (A) Genome browser tracks depicting DNMT3B, OCT1, and H3K36me3 enrichment in occupancy within PITPNC1 and LINC00910 enhancer regions in vehicle-treated (blue, control) and PTS-treated (red) MCF10CA1a breast cancer cells. The data are based on ChIP-seq analyses. The blue-shaded region represents the DNMT3B-increased, OCT1-reduced, and h3K36me3-reduced overlapping peaks. (B) Expression of PITPNC1 and LINC00910, as determined by QPCR in MCF10CA1a breast cancer cells with OCT1 knockdown, which was generated using siRNA. Cells transfected with scrambled siRNA (siCtrl) served as a control. All results represent mean ± SD of three independent experiments; ** p < 0.01, * p < 0.05.