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. 2021 Jul 29;10(8):1219. doi: 10.3390/antiox10081219

Figure 2.

Figure 2

EcSOD protein and mRNA expression are decreased in lung adenocarcinoma, with frequent alterations in EcSOD promoter methylation. (A) Western blot for EcSOD. NL represents normal lung tissue while LT1-LT5 are lung adenocarcinoma tumor samples. The x axis represents the clinical sample labels for both panels A and B. (B) Expression of EcSOD mRNA, as determined by quantitative real-time RT-PCR. Each value is shown as a relative value compared to NL and normalized to 18S transcription level (* p < 0.05 vs. normal). (C) Heat map depicting the percent methylation of CpG sites 6–12 (right y axis) of the EcSOD promoter in NL and LT1-LT5. As depicted in the legend along the left y axis, low methylation is labeled in light yellow and the highest methylation in dark blue. (D) Results of TaqMan copy number variation assay in human lung cancer samples and cell lines. The y axis represents gene copy number, and the x axis represents clinical samples. Error bars, S.D. of each sample run in triplicate [37].