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. 2021 Jul 26;10(8):1190. doi: 10.3390/antiox10081190

Figure 2.

Figure 2

Western blot analysis of left ventricular samples from sham-operated and pressure-overloaded rats with heart failure. (A–E) Relative protein expression of left ventricular (LV) sodium-glucose cotransporter 1 (SGLT1); nicotinamide adenine dinucleotide phosphate (NADPH) oxidase isoform Nox4; phosphorylated extracellular matrix-regulated protein kinase 1 and 2 (P-ERK1/2; Thr202/Tyr204), and total ERK1 and 2 ratio separately and in combination; phosphorylated adenosine monophosphate-activated protein kinase α subunit (P-AMPKα; Thr172) and total AMPKα ratio; and phosphorylated acetyl coenzyme-A carboxylase (P-ACC; Ser79). (F–J) Cropped full-length blots according to the quantification. Following normalization to corresponding housekeeping (GAPDH), protein expression data were normalized to the mean of the respective Sham group to depict fold changes. * p < 0.050, ** p < 0.010, *** p < 0.001.