Figure 1.

Scheme of experimental design. Experiment was started when adherent human monocytes were supplemented with hemozoin (HZ), 4-hydoxynonenal (4-HNE), latex beads (Latex), or RBC. The phagocytosis was completed in 3 h, when non-phagocytosed material was washed out. Monocytes were differentiated to DC during 7 days by supplementation with GM-CSF and IL-4 every 48 h. 4-HNE and GM-CSF/Il-4 were repeatedly added after culture medium changes, as shown in the diagram. Performed analysis and figures which summarise obtained data are indicated at respective points below the timeline.