Figure 5.

Inhibition of surface expression of CD116 in HZ-fed or 4-HNE-treated monocytes. Monocytes were kept as untreated controls (CTRL), fed with neutral latex beads (Latex) or HZ, or treated with different concentrations of 4-HNE as indicated (A,B). The surface expression of CD116 was quantified by flow cytometry after immune staining and acquired as MFI (A) or percentage of GM-CSF R-positive cells (B). Obtained relative MFI and share of CD116-positive treated cells were referred to the respective values measured in untreated control cells from the same donor. Means ± SE of experiments made with monocytes from 3–8 donors. The significances of differences between unfed control and treated cells: ** for p < 0.05, * for p < 0.01.