Figure 6.

Inhibition of expression of CD116 in whole cell lysate of HZ-fed or 4-HNE-treated monocytes. Protein expression of CD116 was analysed in 20 µg lysate protein from monocytes harvested at day 1 after HZ phagocytosis or 4-HNE treatment. Proteins were separated by one-dimensional SDS-PAGE and Western blotted. Transferred proteins were probed with specific primary anti-CD116 and with anti-actin antibodies, the latter for quality control of transfer and protein load per lane. Bound antibodies were detected by binding of appropriate secondary antibodies and evidenced by ECL. One representative experiment of 3 (A) and densitometric analysis (B) are shown. Values are referred to controls and expressed as a percentage. Means ± SE of 3 independent experiments from 3 different phagocyte donors are shown. Significant differences are indicated as * p < 0.01.