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. 2021 Aug 5;10(8):1250. doi: 10.3390/antiox10081250

Table 3.

Sequences and relative positions of primers for target genes used in real-time qPCR.

Gene Sequence Amplicon bp Accession No. * References
GAPDH F: 5′-GCTGGCATTGCACTGAATGAC-3′ 113 NM_204305.1
R: 5′-CACTCCTTGGATGCCATGT-3′
ACTB F: 5′-AGCGAACGCCCCCAAAGTTCT-3′ 139 NM_205518.1 [31]
F: 5′-AGCTGGGCTGTTGCCTTCACA-3′
CAT R: 5′-TGGCGGTAGGAGTCTGGTCT-3′ 112 NM_001031215.1 [32]
R: 5′-GTCCCGTCCGTCAGCCATTT-3′
GPX1 F: 5′-AACCAATTCGGGCACCAG-3′ 122 NM_001277853.2
R: 5′-CCGTTCACCTCGCACTTCTC-3′
GPX2 F: 5′-GAGCCCAACTTCACCCTGTT-3′ 75 NM_001277854.2
R: 5′-CTTCAGGTAGGCGAAGACGG-3′
SOD1 (CuZn) F: 5′-CACTGCATCATTGGCCGTACCA-3′ 224 NM_205064.1 [33]
R: 5′-GCTTGCACACGGAAGAGCAAGT-3′
GSTA2 F: 5′-GCCTGACTTCAGTCCTTGGT-3′ 138 XM_015284825.3 [33]
R: 5′-CCACCGAATTGACTCCATCT-3′
NOS2 F: 5′-AAAGAAAGGGATCAAAGGTGGT-3′ 296 NM_204961.1 [34]
R: 5′-CAAGCATCCTCTTCAAAGTCTG-3′
NOX1 F: 5′-TCATCACTCTGGCGCTCATC-3′ 171 XM_040698828.1
R: 5′-CCTTCATGCTCTCCTCCGTC-3′
NOX2 F: 5′-TGGTGCGGTTTTGGAGATCA-3′ 145 XM_040698636.1
R: 5′-GACACTGCTGGGCATTTGAC-3′
NOX3 F: 5′-TTGGAATGGGAGAAGGCCAC-3′ 92 XM_040667279.1
R: 5′-AGCACCACAGGACTCACAAC-3′

* Ref Seq: NCBI Reference Sequence database.