Figure 1.

NaIO₃ upregulates ferritin in ARPE-19 cells without altering other iron modulating proteins. (A) Probing of lysates from ARPE-19 cells exposed to 10 mM NaIO₃ for 24 h for ferritin revealed significant upregulation relative to vehicle treated controls (lanes 2, 4, 6 vs. 1, 3, 5). Re-probing of the same membrane for transferrin receptor (TfR) showed no significant change in their levels between the experimental and control groups (lanes 2, 4, 6 vs. 1, 3, 5). (B) Densitometry after normalization with β-actin showed a 10-fold increase in ferritin and minimal change in TfR by NaIO₃ treatment relative to controls. (C) Probing of lysates from a similar experimental setup for pro-hepcidin and Fpn showed no significant change in their levels by NaIO₃-treatment relative to controls (lanes 2, 4, 6 vs. 1, 3, 5). (D) Densitometry after normalization with β-actin shows non-significant changes in the levels of pro-hepcidin and Fpn between experimental and control samples. Full blots and their details are provided in Supplementary Figure S1. Values are means ± SEM of the indicated n. *** p ≤ 0.001.