Table 3.

Phenolic compounds putatively identified in the fresh, oven-dried, and freeze-dried extracts from S. ramosissima.

Peak	tr (min.) a	λmax (nm)	Precursor Ion [M-H]− m/z	Product Ion m/z	Tentative Identification	Extracts b	References
1	7.14	301	215	191, 179	quinic acid derivative	FH	[69,70]
2	8.58	275	133	133, 115, 113, 71	malic acid	OD	[70,71,87]
3	9.36	260	191	111, 87, 85	quinic acid	FH, OD, FD	[69,70]
4	29.03	300,325	353	191, 179, 135	neochlorogenic acid	FH, OD, FD	[71,72,88]
5	32.77	280,317	285	153, 152, 108, 109	protocatechuic-acid-arabinoside	FH	[69,89]
6	33.83	275	305	305, 225, 97, 59	gallocatechin	FH, FD	[90]
7	34.85	284,318	355	137, 93	salicylic acid derivative	FH, OD	[91]
8	35.38	281,332	355	273, 253, 191, 173	hydrocaffeoylquinic acid	FD	[15]
9	35.63	274, 310	163	119	p-coumaric acid	FH, FD	[78,92]
10	36.58	300,327	353	191, 179	chlorogenic acid *	FH, OD, FD	[71,72,88]
11	38.63	267,345	193	134, 161, 178	ferulic acid	FH, OD, FD	[69,88]
12	40.26	269,332	355	193, 178, 161, 134	ferulic acid-glucoside	FH, OD, FD	[69,87,88]
13	40.69	268,337	303	303, 97	dihydroquercetin (taxifolin)	FH, OD, FD	[93,94]
14	42.40	312	337	191, 173, 163	p-coumaroylquinic acid (isomer 1)	FH, OD, FD	[95,96]
15	43.88	-	371	249, 121, 113	saccharide	FH, OD, FD	[97]
16	44.60	311	337	215, 191, 173, 163	p-coumaroylquinic acid (isomer 2)	OD	[95,96]
17	45.49	256,336	319	295, 294, 187, 97	n.i.	FH, FD
18	46.34	270,354	609	301, 151	quercetin-rhamnosyl-hexoside	FH, OD, FD	[98]
19	46.50	300	449	253, 118	p-coumaric acid benzyl ester derivative	OD	[99]
20	47.10	274,335	519	315, 301, 300, 299	quercetin-methyl-ether derivative (isomer 1)	FH, FD	[100]
21	47.64	255,352	463	463, 301, 300	quercetin 3-glucoside*	FH, OD, FD	[15,71,78]
22	48.18	284,329	517	517, 355, 179, 135	caffeic acid-glucuronide-glucoside (isomer 1)	FD	[88,101]
23	48.62	251,358	549	505, 463, 301, 300	quercetin-malonyglucoside	FH, OD, FD	[71,84,102]
24	49.37	302,332	515	353, 325, 191, 179	3,5-dicaffeoylquinic acid	FH, OD, FD	[70,96]
25	50.38	302,329	515	479, 353, 191, 179, 173	4,5-dicaffeoylquinic acid	FH, OD, FD	[70,78,96]
26	50.94	266,331	517	355, 179, 135	caffeoyl-hydrocaffeoylquinic acid	FH, OD, FD	[15]
27	51.66	268,346	519	519, 350, 315, 300	quercetin-methyl-ether derivative (isomer 2)	FH, OD, FD	[100]
28	52.33	328	563	503, 473, 459, 443, 383, 353	apigenin-6-arabinosyl-8-glucoside (isoschaftoside)	FH	[94,103]
29	59.90	287	953	953, 767, 575, 285	kaempferol derivative	FH, FD	[71,104]

^a Mean value for retention times obtained from the analysis of fresh, oven-dried and freeze-dried S. ramosissima extracts. ^b Extracts: FH, fresh; OD, oven-dried; and FD, freeze-dried. * Phenolic compound identified with standard. n.i. means not identified.The phenolic composition of S. ramosissima samples was in compliance with the literature [15,105]. The occurrence of quinic acid, p-coumaric and ferulic acid, 3-O-caffeoylquinic acid, and quercetin-3-O-glucoside in aqueous extracts [105], and quercetin-glucoside, hydrocaffeoylquinic, caffeoylquinic, dihydrocaffeoyl quinic, caffeoyl-hydrocaffeoylquinic acid, and dicaffeoyl quinic acids in an ethyl acetate fraction isolated from S. ramosissima were already described [15]. Quercetin-glucoside, caffeoylquinic acid, and other hydroxycinnamic acid derivatives were also reported in other Salicornia species [19,106,107].