Table 1.
Response surface design with experimental and predicted results.
| Run | Factor X1 Amplitude % (Code) | Factor X2 Time Min (Code) | Factor X3 Solvent/Solid mL/g (Code) | Factor X4 Temp. °C (Code) | PAs (mg PC/g DW) | TPC (mg CAT/g DW) | ||
|---|---|---|---|---|---|---|---|---|
| Experimental | Predicted | Experimental | Predicted | |||||
| 1 | 30 (−1) | 10 (−1) | 40 (+1) | 60 (+1) | 109.36 ± 7.23 | 105.81 | 162.11 ± 6.01 | 161.50 |
| 2 | 40 (0) | 15 (0) | 30 (0) | 50 (0) | 87.32 ± 5.15 | 85.01 | 135.69 ± 5.67 | 132.36 |
| 3 | 30 (−1) | 10 (−1) | 20 (−1) | 60 (+1) | 87.54 ± 4.87 | 84.45 | 131.92 ± 4.17 | 132.14 |
| 4 | 40 (0) | 5 (−2) | 30 (0) | 50 (0) | 80.65 ± 5.1 | 77.69 | 125.18 ± 3.69 | 123.71 |
| 5 | 40 (0) | 15 (0) | 30 (0) | 70 (+2) | 122.19 ± 5.71 | 119.55 | 177.15 ± 7.79 | 176.86 |
| 6 | 40 (0) | 15 (0) | 30 (0) | 50 (0) | 89.54 ± 3.57 | 85.01 | 127.91 ± 3.89 | 132.36 |
| 7 | 40 (0) | 25 (+2) | 30 (0) | 50 (0) | 94.18 ± 3.48 | 92.13 | 141.44 ± 7.70 | 144.40 |
| 8 | 40 (0) | 15 (0) | 50 (+2) | 50 (0) | 103.89 ± 4.18 | 99.67 | 141.37 ± 4.07 | 140.83 |
| 9 | 50 (+1) | 10 (−1) | 20 (−1) | 60 (+1) | 90.19 ± 5.08 | 86.29 | 133.61 ± 3.79 | 132.22 |
| 10 | 30 (−1) | 20 (+1) | 20 (−1) | 40 (−1) | 75.96 ± 3.52 | 71.63 | 120.24 ± 3.34 | 117.37 |
| 11 | 50 (+1) | 20 (+1) | 20 (−1) | 40 (−1) | 82.87 ± 4.11 | 81.07 | 138.44 ± 4.74 | 137.29 |
| 12 | 20 (−2) | 15 (0) | 30 (0) | 50 (0) | 81.73 ± 4.57 | 82.57 | 129.46 ± 4.77 | 132.58 |
| 13 | 50 (+1) | 10 (−1) | 40 (+1) | 60 (+1) | 105.18 ± 5.67 | 102.85 | 159.67 ± 3.93 | 158.42 |
| 14 | 40 (0) | 15 (0) | 30 (0) | 50 (0) | 86.99 ± 4.25 | 85.01 | 137.67 ± 5.15 | 132.36 |
| 15 | 50 (+1) | 10 (−1) | 20 (−1) | 40 (−1) | 74.68 ± 3.81 | 74.95 | 117.81 ± 2.95 | 115.55 |
| 16 | 50 (+1) | 20 (+1) | 40 (+1) | 60 (+1) | 116.42 ± 6.23 | 113.57 | 169.71 ± 5.41 | 168.57 |
| 17 | 50 (+1) | 10 (−1) | 40 (+1) | 40 (−1) | 83.67 ± 4.52 | 80.31 | 124.36 ± 3.72 | 128.91 |
| 18 | 30 (−1) | 20 (+1) | 40 (+1) | 40 (−1) | 84.08 ± 4.61 | 82.79 | 125.28 ± 3.35 | 124.91 |
| 19 | 30 (−1) | 20 (+1) | 40 (+1) | 60 (+1) | 121.11 ± 3.08 | 114.13 | 162.37 ± 5.73 | 160.45 |
| 20 | 40 (0) | 15 (0) | 10 (−2) | 50 (0) | 72.82 ± 4.06 | 71.95 | 105.06 ± 4.13 | 107.09 |
| 21 | 30 (−1) | 20 (+1) | 20 (−1) | 60 (+1) | 93.75 ± 4.35 | 91.77 | 145.66 ± 3.94 | 139.71 |
| 22 | 40 (0) | 15 (0) | 30 (0) | 50 (0) | 86.12 ± 3.56 | 85.01 | 137.27 ± 2.52 | 132.36 |
| 23 | 30 (−1) | 10 (−1) | 20 (−1) | 40 (−1) | 70.59 ± 4.11 | 67.91 | 107.45 ± 4.58 | 106.83 |
| 24 | 30 (−1) | 10 (−1) | 40 (+1) | 40 (−1) | 80.45 ± 4.19 | 78.07 | 127.30 ± 4.18 | 122.99 |
| 25 | 40 (0) | 15 (0) | 30 (0) | 50 (0) | 88.23 ± 5.07 | 85.01 | 127.13 ± 4.32 | 132.36 |
| 26 | 60 (+2) | 15 (0) | 30 (0) | 50 (0) | 95.19 ± 4.23 | 89.05 | 150.96 ± 4.48 | 149.42 |
| 27 | 40 (0) | 15 (0) | 30 (0) | 50 (0) | 88.37 ± 5.15 | 85.01 | 132.66 ± 3.95 | 132.36 |
| 28 | 40 (0) | 15 (0) | 30 (0) | 30 (−2) | 79.37 ± 4.51 | 76.87 | 122.94 ± 4.17 | 124.65 |
| 29 | 50 (+1) | 20 (+1) | 40 (+1) | 40 (−1) | 90.87 ± 4.81 | 87.43 | 146.06 ± 2.99 | 141.67 |
| 30 | 50 (+1) | 20 (+1) | 20 (−1) | 60 (+1) | 100.07 ± 6.28 | 96.01 | 150.82 ± 3.16 | 150.99 |