Figure 5.

Loss of striatonigral integrity in the Ift88 cKO and in the MPTP models is associated with increased length of PC in striatal neurons. Representative confocal images of dorso-lateral striatal cryosections showing PC immunostained with an antibody recognizing ACIII (green) in NeuN positive neuronal nuclei (red) in control and Ift88 cKO mice (A,B) and in MPTP-treated and not treated controls (D,E). Quantification of the average PC length in dorso-lateral striatum in control and IftI88 cKO mice (C) and in control mice upon MPTP treatment (F). Arrows point to the PC shown in the inset. (G–J) Representative confocal images of ventral midbrain cryosections showing PC stained with an antibody recognizing ACIII (green) in TH positive neurons (red) of the SN and VTA in control mice non-treated and treated with MPTP. (K,L) Quantification of the average PC length in control mice upon MPTP treatment in SN and VTA. At least 80 cilia from each mouse were measured (n = 3 mice per group). The data are presented as the mean ± SEM. Statistical significance between the indicated groups determined by unpaired two-tailed Student’s t-test, *** p < 0.001 (for details, see also supplementary statistical information). Scale bar: (A,B,D,E,G–J); insets: 12 μm.