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. 2021 Aug 20;10(8):1311. doi: 10.3390/antiox10081311

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cerebral occludin and ZO-1 protein expression. Occludin, ZO-1 and β-actin representative immunoblots from mice brain tissue and their quantification. (A) Cerebral occludin protein levels expressed as a percentage of the optical density (% OD) in the aging mice vs. the Control Young group. ** p < 0.01, **** p < 0.0001 vs. Control Young and ^# p < 0.05 vs. Aged. (B) Cerebral ZO-1 protein levels expressed as a percentage of the optical density (% OD) in the aging mice vs. the Control Young group. ***p < 0.001 vs. Control Young and ^# p < 0.05 vs. Aged. n = 13–19. The results are expressed as the mean ± SEM. Control Young: young mice, Aged: CRE^+/− aging mice and Aged NOX5: NOX5^+/−CRE^+/− aging mice.