Table 1.
The primers used for cDNA amplification.
| Gene | Accession Number | Primers | |
|---|---|---|---|
| NOX2 | NM_007807.5 | forward | 5’-ACTCCTTGGGTCAGCACTGG-3’ |
| reverse | 5’-GTTCCTGTCCAGTTGTCTTCG-3’ | ||
| NOX4 | NM_001285833.1 | forward | 5’-GGAGACTGGACAGAACGATTCC-3’ |
| reverse | 5’-TGTATAACTTAGGGTAA TTTCTAGAGTGAATGA-3’ |
||
| NOX5 | NM_001184780.2 | forward | 5’-ATGAGTGCCGAGGAGGATG-3’ |
| reverse | 5’-ATCGATGGCAGTGGCTCCAT-3’ | ||
| CATALASE | NM_009804.2 | forward | 5’-GCTGAGAAGCCTAAGAACGCAAT-3’ |
| reverse | 5’-CCCTTCGCAGCCATGTG-3’ | ||
| eNOS | NM_008713.4 | forward | 5’-CTGGAGCACCCCACGCT-3’ |
| reverse | 5’-AGCGGTGAGGGTCACACAG-3’ | ||
| p22phox | NM_007806 | forward | 5’-GCCCTCCACTTCCTGTT-3’ |
| reverse | 5’-GCAGATAGATCACACTGGCAAT-3’ | ||
| SOD2 | NM_013671 | forward | 5’-CACACATTAACGCGCAGATCA-3’ |
| reverse | 5’-GGTGGCGTTGAGATTGTTCA-3’ | ||
| COX1 | NM_008969.4 | forward | 5’-ACTCACAGTGCGGTCCAAC-3’ |
| reverse | 5’-AACTCCCTTCTCAGCAGCAG-3’ | ||
| COX2 | NM_011198.4 | forward | 5’-TTCGGGAGCACAACAGAGT-3’ |
| reverse | 5’-TAACCGCTCAGGTGTTGCAC-3’ | ||
| PGES | NM_022415.3 | forward | 5’-AGGATGCGCTGAAACGTGGAG-3’ |
| reverse | 5’-CCGAGGAAGAGGAAAGGATAG-3’ | ||
| TXA2S | NM_011539.3 | forward | 5’-AACAGAATGGCCTCAGGTCT-3’ |
| reverse | 5’-AGTTCACAGGCTTGGCTGAT-3’ | ||
| OCCLUDIN | NM_001360536.1 | forward | 5’-CGGCAGGTTCGCTTATCTT-3’ |
| reverse | 5’-TGTCATTGCTTGGTGCATAA-3’ | ||
| ZO-1 | NM_009386.2 | forward | 5’-CCGCTAAGAGCACAGCAAT-3’ |
| reverse | 5’-CATTGCAACTCGGTCATTTT-3’ | ||
| GAPDH | NM_008084.3 | forward | 5’-ATGACAACTTTGTCAAGCTCATTT-3’ |
| reverse | 5’-GGTCCACCACCCTGTTGCT-3’ |
Endothelial nitric oxide synthase (eNOS), Superoxide dismutase 2 (SOD2), Cyclooxygenase 1 (COX1), Cyclooxygenase 2 (COX2), Prostaglandin E synthase (PGES) and Thromboxane A2 synthase (TXA2S).