Figure 2.

Synthesis and characterization of TAT-HA-Prdx6^WT, TAT-HA-Prdx6^K122/142R, and TAT-HA-Prdx6^{Inactive-mutant} protein-loaded nanoparticles. (A) Schematic representation of TAT-HA-Prdx6 protein analog-loaded nanoparticles made by the emulsion solvent evaporation technique [61,74]. (B) Zeta potential of TAT-HA-Prdx6^WT-NPs, TAT-HA-Prdx6^K122/142R-NPs, and TAT-HA-Prdx6^{Inactive-mutant}-NPs before and after lyophilization. Surface charge (zeta potential) of encapsulants prepared by mixing 1:50 ratio of TAT-HA-Prdx6 protein analog-NPs with DH₂O. Standard error (mean ± SD) for n = 3. (C) Representation of atomic force microscopy (AFM) images showing homogenous particle distribution. AFM image (inset, image after dilution) and corresponding size (diameter—nm) distributions of TAT-HA-Prdx6 protein analog NPs.