Figure 9.

Curcumin inhibits TRPM2 non-selective cation channels, assayed by patch clamp recording, in isolated rat hepatocytes and in HEK293T cells heterologously expressing TRPM2. (A) Current–voltage plots for hepatocytes treated with H₂O₂ to activate TRPM2, measured in the presence and absence of 5 µM curcumin. (B) Average amplitude of the TRPM2-mediated Na⁺ current in untreated hepatocytes (Control), hepatocytes treated with H₂O₂ (H₂O₂) and in hepatocytes treated with H₂O₂ in the presence of 5 µM curcumin (H₂O₂ + Curcumin). ** P < 0.008 (C) Dose–response curves for the inhibition of the TRPM2 current activated by ADP-ribose (two concentrations administered via the patch pipette) to HEK293T cells heterologously expressing TRPM2. The IC₅₀ values for curcumin inhibition are about 50 nM. Reproduced with permission from [119], copyright 2016 Elsevier.