FIG. 1.

Genetic map of MVM. The three major transcript classes and protein-encoding ORFs are shown. The two promoters (P4 and P38) are indicated by arrowheads. The large intron, small intron, and NS2-specific exon are indicated. The nonconsensus donor (ncD) and the poor polypyrimidine tract [poor (Py)n] of the large intron are also shown. The bottom diagram shows nucleotide locations, the two probes (A [nt 385 to 650] and B [nt 1854 to 2378]) used for RNase protection assays, and the two primers [a (nt 326 to 345) and b (nt 2557 to 2538)] used for RT-PCR, as described fully in Materials and Methods.