Skip to main content
. 2021 Aug 26;16(8):e0256646. doi: 10.1371/journal.pone.0256646

Fig 2. RhoGDI2 knockdown in CRIF1-silenced HUVECs leads to activation of the Akt-CREB signaling pathway and restoration of endothelial cell migration.

Fig 2

(A) HUVECs were transfected with the control, CRIF1 siRNA, or co-transfected with CRIF1 and RhoGDI2 siRNA (100 pmol) for 48 h followed by western blot analysis of phospho-Akt and phospho-CREB levels. (B) Protein levels were quantified by densitometric analysis using ImageJ software. (C) HUVECs were transfected with the control, CRIF1 siRNA, or co-transfected with CRIF1 and RhoGDI2 siRNA (100 pmol) and incubated for 24 h. Next, the cells were wounded for 24 h. Images were obtained using a light microscope. (D) Quantification of wound closure was performed using ImageJ software. (E) HUVECs were transfected with the control, CRIF1 siRNA, or co-transfected with CRIF1 and RhoGDI2 siRNA (100 pmol) and transwell assay was conducted to determine cell migration. Scale bar 200 μm. (F) Quantification of the number of migrated cells was performed using ImageJ software. Data are means ± SD of three independent experiments. **P < 0.01 and *** P < 0.001 relative to the control, #P < 0.05, ##P < 0.01 and ### P < 0.001 relative to siCRIF1 (n = 3 per group).