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. 2021 Aug 26;16(8):e0256646. doi: 10.1371/journal.pone.0256646

Fig 5. Effect of exogenous ADM2 on the migration of CRIF1-silenced endothelial cells.

Fig 5

(A) HUVECs were transfected with control or CRIF1 siRNA (100 pmol) and treated with ADM2 for 24 h. Next, the cells were wounded for another 24 h. Images were obtained using a light microscope. (B) Quantification of wound closure was performed using ImageJ software. (C) HUVECs were transfected with control or CRIF1 siRNA (100 pmol), treated with ADM2 for 24 h.and transwell assay was conducted to determine cell migration. Scale bar 200 μm. (D) Quantification of the number of migrated cells was performed using ImageJ software. Data are means ± SD of three independent experiments. **P < 0.01 and *** P < 0.001 relative to the control, ##P < 0.01 relative to siCRIF1 (n = 3 per group).