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. 2021 Aug 26;17(8):e1009800. doi: 10.1371/journal.ppat.1009800

Fig 2. Suppression of MAVS induced IFNβ-luciferase promotor activation by SARS-CoV-2 genes.

Fig 2

HEK-293T cells were co-transfected with IFNβ-firefly luciferase, Renilla luciferase, MAVS and a SARS-CoV-2 viral genes. ’No trigger’ refers to cells transfected with Firefly and Renilla only (but no MAVS); ’Trigger only’ refers to cells transfected with all components but without a viral gene. 24 hours post transfection luciferase activity was measured. IFNβ-firefly (A), Renilla normalization signal (B) and normalized Firefly/Renilla luciferase (C) activities are presented separately. Data presented are means pooled from four independent experiments and their standard errors. Significance values are given in comparison to the GFP positive control (green bar): * P < 0.05. Black bars in the plot: 10-fold or greater repression of MAVS-induced promoter activity in comparison to the GFP control. Grey bars in histograms: Minor (< 10-fold) repression of MAVS-induced IFNβ activity.