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. 2021 Aug 26;17(8):e1009800. doi: 10.1371/journal.ppat.1009800

Fig 4. Suppression of MAVS induced IFNβ, IFNλ1 and IFNλ2/3 gene expression by SARS-CoV-2 genes determined by qPCR.

Fig 4

HEK-293T cells were transfected with MAVS and a SARS-CoV-2 viral gene or GFP. 24 hours post transfection, transcript levels were analyzed by qPCR. The data presented are expression levels normalized to housekeeping gene HPRT1 (ΔCT). Results are shown for (A) IFNβ; (B) IFNλ1; and (C) IFNλ2/3 (common primers amplify both IFNλ2 and IFNλ3). Data presented are means of 2–4 independent experiments ± SEM. Significance was compared to the GFP positive control: * P < 0.05, ** P < 0.001, *** P < 0.0001.