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HEK-293T cells were transfected with a SARS-CoV-2 viral gene, or GFP control. 24 hours post transfection cells were treated with 100 pM IFNβ for an additional 24 hours before harvest. Transcript levels of four ISGs were analyzed by qPCR. The data presented are expression levels normalized to housekeeping gene HPRT1 (ΔCT). Average values ± SEM were calculated based on 2–3 independent experiments.
