Table 1. Result of the qPCR assay and culture for Acanthamoeba spp. in various waters sources.
| Water sources | Sample No. | Culture | qPCR | Mean Ct value | Acanthamoeba spp. (plasmid copies/l) | ||
|---|---|---|---|---|---|---|---|
| N | (%) | N | (%) | ||||
| StW | 9 | 4 | 44.44 | 4 | 44.44 | 32.9 | 8.8x104-7.3x102 |
| LW | 18 | 2 | 11.11 | 5 | 27.77 | 37.2 | 1.2x103-1.4x102 |
| SeW | 8 | 2 | 25 | 2 | 25 | 31.2 | 3.2x105-5.3x103 |
| PW | 26 | 3 | 11.53 | 6 | 23.07 | 36.8 | 5.3x103-1.6x102 |
| WW | 31 | 4 | 12.9 | 6 | 19.35 | 34.4 | 1.6x104-1.4x103 |
| TW | 44 | 3 | 6.81 | 4 | 9.09 | 36.1 | 1.9x104-1.8x102 |
| Total | 136 | 18 | 13.23 | 27 | 19.85 | ||
TW: Tap water, PW: Pool water, WW: Well water, LW: Lake water, StW: Stream water, SeW: Seawater