Western blot analysis of IL-1β, TNFα, HMGB1, and NLRP3 in mice. (a) Western blot bands about IL-1β, TNFα, HMGB1, and NLRP3. (b) The relative expression of IL-1β/β-actin at the protein level. (c) The relative expression of TNFα/β-actin at the protein level. (d) The relative expression of HMGB1/β-actin at the protein level. (e) The relative expression of NLRP3/β-actin at the protein level. *Compared with control group, P < 0.05. #Compared with the KA group, P < 0.05.