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The primer sequence of gene
| Gene | Primer sequence (5′ → 3′) |
|---|---|
| IL-1β | FOR: TGTGATGTTCCCATTAGAC |
| REV: AATACCACTTGTTGGCTTA | |
| HMGB1 | FOR: GGCGGCTGTTTTGTTGACAT |
| REV: ACCCAAAATGGGCAAAAGCA | |
| NLRP3 | FOR: CACCTCTTCTCTGCCTACCTG |
| REV: AGCTGTAAAATCTCTCGCAGT | |
| Occludin | FOR: CCCAGACCACTATGAAACCGACT |
| REV: CAGCCATGTACTCTTCGCTCT | |
| TNF-α | FOR: CCCCTCTATTTATAATTGCACCT |
| REV: CTGGTAGTTTAGCTCCGTTT | |
| Claudin5 | FOR: TGGCACTCTTTGTTACCTTGACC |
| REV: ACCGTTGGATCATAGAACTCCC | |
| β-Actin | FOR: ACATCCGTAAAGACCTCTATGCC |
| REV: TACTCCTGCTTGCTGATCCAC |
