Figure 4.

(a) Conformational rearrangement from closed isolated Fas_DD (pdb-id: 1DDF, green) to open bound Fas_DD (pdb-id: 3EZQ, red). (b) Fas_DD opening discloses a hydrophobic patch that is the binding site of FADD_DD, shown with dashed circles. (c) Fas_DD rearrangement allows for homodimerization of two open Fas molecules through interactions between their stem helices in a Fas–Fas bridge conformation. (d) Kelley penalty plot and (e) distance matrix from Fas_DD–Fas_DD clustering. (f) Most populated cluster with 83 members. Fas_1,DD and Fas_2,DD are presented in green and cyan colors, respectively. (g) Predicted complex (red) superposed on the crystal structure (green) (pdb-id: 3EZQ) with a Cα RMSD value of 1.1 Å.