Figure 6.

(a) Multiple sequence alignment of residues that form part of the hydrophobic patches in the α1/α4 and α2/α5 surfaces of FADD_DED, C8_DEDs, and cFLIP_DEDs. H/Y residues in α1, the FL motif in α2, and the RxDL motif in α5 are highlighted. Maestro Schrodinger 2020-2 was used for the alignment. (b) PCA results for intersubdomain interactions of C8_DED1 and C8_DED2 with cFLIP_DED1 and cFLIP_DED2. C8_DEDs and cFLIP_DEDs domains were coexpressed in HEK293T as indicated. Negative control is the result of coexpression of empty vectors (containing only a subunit of the luciferase as indicated). The luminescence is expressed in arbitrary unit (AU). Data represent mean ± SD of three independent experiments for each cotransfection.