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. 2021 Jul 28;9(8):904. doi: 10.3390/biomedicines9080904

Figure 2.

Figure 2

Sirtuin 6 (SIRT6) levels in pericoronary fat patients. (A,B) Sirt6 protein level in pericoronary fat specimens from 150 normal glucose, 150 pre-diabetic patients, and 55 pre-diabetic current-metformin-users detected by western blotting analysis. Lane 1 = molecular markers; Lane 2 = normal glucose patients; lane 3 = pre-diabetic patients; lane 4 = pre-diabetic patients with metformin. The expression level was normalized with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as internal control using Image J software and values expressed as arbitrary units (AU). (C) SIRT6 level in pericoronary fat specimens from 150 normal glucose, 150 pre-diabetic patients and 55 pre-diabetic current-metformin-users assessed by ELISA assay on homogenates. Boxplots represent the median, 25th and 75th percentiles values while the black symbols outside the 10th and 90th percentiles. For comparison between the cohorts we used the ANOVA test. * p < 0.05 vs. normal glucose patients; ‡ p < 0.05 vs. pre-diabetic patients.