Figure 3.

Amorfrutin B (5 μM) post-treatment reduced the oxidative stress resulting from hypoxia and ischemia in neuronal cells. There was an increase in reactive oxygen species formation and 8-OHdG in the hypoxic and ischemic models, and amorfrutin B diminished the activity of these markers in both cases (a,b). The ROS activity results are presented as a percentage of the control ± SEM, and the 8-OHdG level results are presented as the mean ± SEM. There were 3 independent experiments, consisting of 10–12 replicates per group. *** p  <  0.001 compared to the control group, ### p < 0.001 compared to the cultures exposed to hypoxia, ^^^ p < 0.001 compared to the cultures exposed to ischemia.