TABLE 1.
Yeast strains used in this study
| Strain | Genotypea | Source |
|---|---|---|
| 1906 | MATa leu2 mak8-1 K− MKT+ | R. Wickner |
| 5X47 | MATa/MAT α his1/+ trp1/+ ura3/+ K−R− | R. Wickner |
| 2373 | MATa ura3 ski4-1 mkt1 K++ | R. Wickner |
| 2898 | MATa ura3 ade3 his(5,6) ski6-2 K++ | R. Wickner |
| 2413 | MATa ura3 cyh2 ski7-2 K++ | R. Wickner |
| JD100 | MATa ura3-52 his3 trp1-δ1 K1+ | This study |
| JD973 | MATα ura3-SK1 LEU2::hisG TRP1::hisG lys2-SK1 ho::LYS2 ade3-210S | This study |
| Cross JD980 | JD100 × JD973 with RPL3::hisG on 1 chromosome | This study |
| JD980-10C | MATα lys2 his3 ura3 LEU2::hisG trp1-δ1 RPL3::hisG + pRPL3 or pmak8-1 | This study |
| JD13 | MATa his3 leu2 PEP4::HIS3 NUC1::LEU2 ura3 K1+ | This study |
| JD111 | MATα ura3-52 lys2-801 trp1-δ1 leu2−− his3−− K1+ | This study |
| JD890 | MATα ura3-52 trp1 leu2Δ1 his3Δ300 pbrΔ1-6 can1r pep4::HIS3 SKI1::LEU2 [L-AHN M1] K++ | This study |
| JD2 | MATα ura3 trp1 ade8 ski2-2 K++ | This study |
a
−−, molecularly undefined allele that at the genetic level does not revert, i.e., is a very strong mutant; ++, phenotypically strong killer strain.