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. 2021 Jul 30;9(8):923. doi: 10.3390/biomedicines9080923

Figure 3.

Figure 3

Involvement of PKC-δ in BK-induced RBA morphological change into neuron-like cells. (A) RBA cells were pretreated with rottlerin (1 μM) for 1 h and then incubated with or without BK (10 nM) for 24 h. (B) Cells were treated without (control) or with PMA (1 μM) for 24 h. (C) Cells were pretreated with rottlerin (10 μM) for 1 h before exposure to 1 μM PMA for 24 h. The phase-contrast images were obtained ((B), scale bar = 20 μm) and the length of neurite fiber outgrowth at 24 h was quantified (A,C). The image represents one of at least three individual experiments (B). Data are expressed as the mean ± SEM of at least three independent experiments. ** p < 0.01, as compared with control. ## p < 0.01, as compared with BK or PMA alone.