Skip to main content
. 2021 Jul 30;9(8):923. doi: 10.3390/biomedicines9080923

Figure 6.

Figure 6

BK upregulates the neuronal marker NSE expression in RBA cells. (A) Time dependence of BK-induced upregulation of NSE protein expression. Cells were treated with 10 nM BK for the indicated times. (B) The upregulation of NSE by BK was confirmed by immunofluorescence in RBA cells. Cells were incubated without (control) or with BK (10 nM) for 24 h and then labeled using an anti-NSE antibody and a FITC-conjugated secondary antibody. Individual cells were imaged (scale bar = 20 μm) as described in ‘‘Methods’’. (C) Cells were pretreated with Hoe140 (Hoe, 10 μM), rottlerin (Rott, 1 μM), PD98059 (PD, 10 μM) for 1 h and then incubated with BK (10 nM) for 24 h. The whole-cell lysates were analyzed by Western blot using an anti-NSE or anti-GAPDH antibody as described under ‘‘Methods’’. Data are expressed as the mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01, as compared with control. ## p < 0.01, as compared with BK alone. The image represents one of at least three individual experiments.