Figure A1.

BK induces neuronal nuclear protein (NeuN) expression in RBA. The upregulation of NeuN by BK was confirmed by immunofluorescence in RBA. Cells were pretreated with Hoe140 (Hoe, 10 μM) for 1 h and then incubated with BK (10 nM) for 24 h. After treatment, the cells were labeled using an anti-NeuN antibody and a FITC-conjugated secondary antibody. Individual cells were imaged (scale bar = 20 μm) as described in ‘‘Methods’’. The fluorescence intensity (arbitrary units: a.u.) of NeuN was quantified in these cells. * p < 0.05, as compared with control. ^# p < 0.05, as compared with BK alone.