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SHH pathway activation is related to CX43 increased expression levels in U-251 MG. (a–c) CX43 and GLI1 expression levels (a,b) and representative blots (c) on human U-251 MG cells exposed to 1 µM of purmorphamine and/or 1 µM cyclopamine. Data are mean FC over control ± SEM of n = 3 independent experiments; * p-value < 0.05, ** p-value < 0.01 versus control or between groups; one-way ANOVA with Holm–Šídák post-hoc test. FC: fold change.
