FIG. 4.

The EBF binding sites are important for the function of the B29 promoter in pre-B cells. The resulting activities of wild-type (B29) and EBF-mutated (B29M) B29 promoter constructs relative to that of a basic luciferase vector (pGL3) after transient transfections into transformed mouse cells of the B-cell lineage are shown. The data are collected from four transfections and two independent experiments from the following cell lines: 230–238 pre-B cells, 18.81 pre-B cells, A20 mature B cells, S194 plasma cells, and J558 plasma cells.