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. 2021 Aug 7;10(8):758. doi: 10.3390/biology10080758

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of cell death induced by Z_HER2:2891DCS-MMAE and trastuzumab treatments. The effect of cellular death induced by Z_HER2:2891DCS-MMAE and trastuzumab was tested in SK-BR-3 (A,C) and MDA-MB-231 cells (B) by flow cytometry. The top left quadrant represents the percentage of early apoptotic cells (Annexin V⁺/PI⁻), the top right quadrant is the percentage of late apoptotic cells (Annexin V⁺/PI⁺); whereas the bottom left quadrant represents the percentage of live cells (Annexin V⁻/PI⁻) and the bottom right quadrant represents the percentage of necrotic cells (Annexin V⁺/PI⁺). Cells were treated with the substances for 10 min followed by drug removal and an additional 48 h of incubation in medium alone (black bar) or for 48 h of continuous exposure to treatments (blue bar). The p-value was determined by one-way ANOVA test with Dunnett post hoc test and considered significant for p < 0.05 *, p < 0.001 *** compared to the control. Data are presented as mean ± SEM.

Detection of cell death induced by Z_HER2:2891DCS-MMAE and trastuzumab treatments. The effect of cellular death induced by Z_HER2:2891DCS-MMAE and trastuzumab was tested in SK-BR-3 (A,C) and MDA-MB-231 cells (B) by flow cytometry. The top left quadrant represents the percentage of early apoptotic cells (Annexin V⁺/PI⁻), the top right quadrant is the percentage of late apoptotic cells (Annexin V⁺/PI⁺); whereas the bottom left quadrant represents the percentage of live cells (Annexin V⁻/PI⁻) and the bottom right quadrant represents the percentage of necrotic cells (Annexin V⁺/PI⁺). Cells were treated with the substances for 10 min followed by drug removal and an additional 48 h of incubation in medium alone (black bar) or for 48 h of continuous exposure to treatments (blue bar). The p-value was determined by one-way ANOVA test with Dunnett post hoc test and considered significant for p < 0.05 *, p < 0.001 *** compared to the control. Data are presented as mean ± SEM.