Table 2. Proteomic Studies in Barleya.
| plant material | technique | number of identified spots/proteins | quantitation | ref |
|---|---|---|---|---|
| leaf | 2-DE | 29 | (117) | |
| seed and malt | 2-DE and MS | 27 for seeds, 3 for malt | (121) | |
| seed | 2-DE and MS | 19 | (122) | |
| seed and malt | 2-DE and MS | 62 in total | (123) | |
| seed | 2-DE and MS | 250 | yes | (124) |
| seed, malt, and beer | 2-DE and LC-MS/MS | 40 for seed, 41 for malt, 30 for beer | (125) | |
| seed | 2-DE and MS | 48 | (126) | |
| seed | 2-DE and MS | 48 | (127) | |
| malted beer | 2-DE and LC-MS/MS | 85 | (128) | |
| wort | 2-DE and MS | 63 | (129) | |
| seed, germinated grain, green malt and malt | 2-DE, MALDI-TOF/TOF MS | 6 (focus on hordeins) | yes, iTRAQ | (130) |
| beer | LC-MS/MS | 33 | yes, emPAI | (131) |
| flour, wort, and beer | LC-MS/MS | 144 in flour, 27 in wort, 79 in beer | yes, MRM | (111) |
| barley and malt | 2-DE, MALDI-TOF/TOF MS | 12 in barley, 9 in malt | (132) | |
| seeds and breakfast products | LC-MS/MS | 96 | yes, MRM | (133) |
| seed | 2-DE and MS | 23 | (134) | |
| leaf | 2-DE and LC-MS/MS | 9258 | yes, intensity | (135) |
| grain | 2-DE, LC-MS/MS | 136 DE spots | (136) | |
| grain | 2-DE, LC-MS/MS | 63 DE proteins | (137) | |
| seeds | LC-MS/MS | 1168 | yes, SPC | (50) |
| seeds | LC-MS/MS | focus on prolamin oxidation | yes, MRM | (138) |
| malt | LC-MS/MS | 1418 | yes, SPC | (139) |
| wort and beer | LC-MS/MS | 210 | yes, SWATH | (73) |
| seed | LC-MS/MS | 220 | yes, SWATH | (140) |
| spent grain | 2-DE, LC-MS/MS | 1346 | yes, intensity | (141) |
| leaf | LC-MS/MS | 1800 | yes, TMT | (142) |
| wort | LC-MS/MS | 87 | yes, SWATH | (143) |
| grain | LC-MS/MS | 1483 | (144) | |
| seeds | LC-MS/MS | 6 (focus on ATIs) | yes, MRM | (145) |
| grain | 2-DE, LC-MS/MS | hordein accumulation | (49) | |
| malt rootlet | LC-MS/MS | 2111 | (146) | |
| wort | 2-DE, LC-MS/MS | protein Z4 and Z7 analysis | (147) | |
| leaf | LC-MS/MS | 6921 | yes, DIA | (148) |
| grain | LC-MS/MS | 1907 | yes, SWATH | (149) |
a
Abbreviations: ATIs - α-amylase trypsin inhibitors, 2-DE - two2-dimensional electrophoresis, emPAI - exponentially modified protein abundance index; iTRAQ - isobaric tags for relative and absolute quantitation; LC - liquid chromatography; MALDI - matrix-assisted laser desorption ionization; MRM - multiple reaction monitoring; MS - mass spectrometry; SPC - spectral counting; SWATH - sequential Window Acquisition of all Theoretical fragmentation spectra; TMT - tandem mass tag, TOF - time-of-flight.