Table 3. Proteomic Studies on Abiotic and Biotic Stress Response in Barley169a.
| plant material | tissue | methods | quantitation | ref | |
|---|---|---|---|---|---|
| Drought | |||||
| barley cv. Basrah (T), Golden Promise (S) | leaf, root | 2-DE- MALDI-TOF | yes | (170) | |
| Two Egyptian accessions; 15141 (T), 15163 (S) | leaf | 2-DE- MALDI-TOF | (171) | ||
| barley cv. Golden Promise | leaf | 2-DE-MS | (172) | ||
| spring malting barley cv. Amulet | leaf, crowns | 2-DE, MALDI-TOF/TOF | yes | (173) | |
| spring barley cv. Maresi (T), Syrian breeding line – Cam/B1/CI (T) | leaf, root | 2-DE, MALDI-TOF/TOF | yes | (174) | |
| spring barley cv. Maresi x Syrian breeding line – Cam/B1/CI (T), 100 RILs | leaf. root | MALDI-TOF/TOF | yes | (167) | |
| Low Temperature (Cold/Frost Tolerance) | |||||
| winter barley cv. Luxor (T) | crown, leaf | 2-DE-MALDI-TOF | yes | (175) | |
| spring barley cv. Aths (S) | leaf, root | 2-DE, LC-MS/MS | (176) | ||
| 10 DH lines: DH534 (T), DH602 (T), DH561 (t), DH61 (t), DH435 (t), DH584 (t), DH363 (S), DH575 (S), DH158 (S), DH65 (S) | leaf | 2-DE-MALDI-TOF/TOF | (177) | ||
| Osmotic Stress (Polyethylene Glycol PEG-6000) | |||||
| Tibetan hull-less barley (Hordeum vulgare L. var. nudum) inbred lines, drought-resistant XL (T), drought-sensitive DQ (S) | leaf | LC-MS/MS | yes | (148) | |
| Salinity | |||||
| barley cv. OUK305 (T), OUI743 (S) | root | 2-DE, LC-MS/MS | yes | (178) | |
| barley cv. Morex (T), Steptoe (S) | root | 2-DE, LC- MS/MS | yes | (179) | |
| barley cv. Afzal (T), L-527 (S) | leaf | 2-DE, MALDI-TOF/TOF | yes | (180) | |
| barley cv. Afzal(T), L-527 (S) | leaf | 2-DE, MALDI-TOF/TOF | yes | (181) | |
| barley cv. Morex (T), Steptoe (S) | leaf, root | 2-DE, MALDI-TOF; LC-qTOF MS/MS | yes | (182) | |
| barley cv. DH187 (T), DH14 (S) | seed | 2-DE, MALDI-TOF/TOF | yes | (183) | |
| barley DH population cross TX9425 and NasoNijo two pairs of NILs (N33 (S) and T46 (T), N53 (S), and T66 (T) | leaf, root | 2-DE, MALDI-TOF/TOF | yes | (184) | |
| Combined Stress | |||||
| drought and osmotic stress | barley genotypes 004223 (T), 004186 (S) | leaf | 2-DE MALDI-TOF; LC-MS/MS | yes | (185) |
| drought and heat stress | Arta (T), Keel (T) | leaf | 2-DE, MALDI-TOF/TOF | yes | (186) |
| drought and Piriformospora. indica | barley cv. Golden Promise | leaf | 2-DE, LC-MS/MS | yes | (187) |
| Pathogenes | |||||
| Fungal Pathogen - Fusarium Head Blight (FHB) | |||||
| barley cv. Scarlett (S) | spikelet | 2-DE, LC-MS/MS | yes | (188) | |
| barley cv. Scarlett (S) | seed | 2-DE, MALDI-TOF, MS/MS | yes | (189) | |
| naked barley (Hordeum vulgare ssp. nudum) | seed | 2-DE MALDI-TOF-MS, LC-MS/MS | yes | (190) | |
| Net Blotch −Pyrenophora teres | |||||
| barley cv. La Trobe, Charger, Oxford, Commander, Fairview, Compass | seed | LC-MS/MS, SWATH-MS | yes | (143) | |
| barley cv. Oxford, Commander, Compass, Scope, Shepherd, Flagship | seed | LC-MS/MS, SWATH-MS | yes | (143) | |
| barley cv. Baudin | leaf | 2-DE, LC-MS/MS, SWATH-MS | yes | (191) | |
| Leaf Rust −Puccinia hordei | |||||
| barley cv. LaTrobe, Commander, Compass, Scope, Shepherd, Fathom | seed | LC-MS/MS, SWATH-MS | yes | (143) | |
| Powdery Mildew– Blumeria graminis | |||||
| barley cv. Golden Promise | leaf | LC-MS/MS | (192) | ||
| barley cv. Golden Promise | leaf | LC-MS/MS | yes | (193) | |
a
Abbreviations: 2-DE - two-dimensional electrophoresis; DH - double haploid (line); LC - liquid chromatography; MALDI-TOF/TOF - matrix-assisted laser desorption ionization time-of-flight/time-of-flight (spectrometry); MS, mass spectrometry; qTOF - quadrupole time-of-flight; S - sensitive (genotype); SWATH - sequential window acquisition of all theoretical spectra; T - tolerant (genotype); t - genotype less tolerant than T.