FIG. 6.

FAST-2 activates the A3-luc reporter gene in response to TGF-β (A) Mv1Lu cells were cotransfected with A3-luc and FAST-1 or FAST-2(9-401) together with Smad2 or Smad3 as indicated. Luciferase activity was determined in cell extracts following 24 h with or without TGF-β (0.1 nM) treatment. Luciferase activity is presented as relative light units (RLU). Open bars; control; filled bars, TGF-β-treated cells. Shown are the means of duplicate samples from a typical experiment. (B) Mv1Lu cells were cotransfected with A3-luc and FAST-2(9-401) or FAST-2d(9-342). Luciferase activity was determined after 36 h with or without TGF-β (0.5 nM) treatment. Luciferase activity is presented in the same fashion as in panel A.