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. Author manuscript; available in PMC: 2021 Aug 26.
Published in final edited form as: Arch Biochem Biophys. 2020 Jul 22;691:108482. doi: 10.1016/j.abb.2020.108482

Figure 4:

Figure 4:

Effect of lipid composition of TNAP-proteoliposomes (DMPC, DMPC:Chol 90:10, DMPC:SM 90:10 and DMPC:Chol:SM 80:10:10, mol %) on mineral propagation in the presence of a PS-CPLX nucleator at pH 7.5. The assay was accomplished using a saturating ATP concentration for each lipid composition as determined in the Figure 3 ranging from 10−6 to 10−2 M were used for proteoliposomes composed of DMPC, DMPC:Chol, DMPC:SM and DMPC:Chol:SM, respectively. Enzyme-devoid liposomes were used as control and bars show the increment in the absorbence after 48 h of incubation at 37 °C. All results are expressed as mean± SEM. P<0.05.