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Kinetic parameters for ATP hydrolysis by TNAP reconstituted into DMPC-liposomes in different lipid compositions (mol%).
| Proteoliposomes (mol%) |
||||
|---|---|---|---|---|
| Kinect parameter | DMPC | DMPC:Chol (90:10) | DMPC:SM (90:10) | DMPC:Chol:SM (80:10:10) |
|
| ||||
| Vmax (U/mg) | 833.2±13.4 | 652.3±11.2 | 701.3±13.2 | 993.8±12.5 |
| K0.5 (mM) | 2.70±0.02 | 3.79±0.03 | 0.29±0.07 | 0.33±0.08 |
| nH | 1.3±0.1 | 1.1±0.1 | 1.4±0.1 | 0.6±0.1 |
| kcat/K0.5 (M−1.s−1) | 6.2 ×102 | 3.4 ×102 | 4.7 ×103 | 6.0 ×103 |
