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. 2021 Aug 12;10:e68275. doi: 10.7554/eLife.68275

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Drosophila melanogaster) PkaC1H2 Bloomington Drosophila Stock Center BDSC:4101, RRID:BDSC_4101 FlyBase symbol: Dmel\Pka-C1H2
Genetic reagent (D. melanogaster) PkaC1B3 Bloomington Drosophila Stock Center FBal0033955 Dmel\Pka-C1B3
Genetic reagent (D. melanogaster) PkaC1A13 Bloomington Drosophila Stock Center FBal0033953 Dmel\Pka-C1A13
Genetic reagent (D. melanogaster) UASmPkaC1*(mC*) D.Kalderon DC0
Genetic reagent (D. melanogaster) moodyGAL4 T.Schwabe FBtp0022847 P{moody-GAL4.S}
Genetic reagent (D. melanogaster) repoGAL4 Bloomington Drosophila Stock CenterV.Auld BDSC:7415FBst0007415 P{GAL4}repo
Genetic reagent (D. melanogaster) NrgG305 Bloomington Drosophila Stock CenterW.Chia BDSC:6,844FBal0147727 Dmel\NrgG00305
Genetic reagent (D. melanogaster) UASGFPMoesin D. Kiehart FBtp0017306 UASmRFPMoesin
Genetic reagent (D. melanogaster) UASmRFPMoesin T. Schwabe FBtp0022846 P{UAS-Moe.RFP}
Genetic reagent (D. melanogaster) Gβ13FΔ1-96A F. Matsuzaki FBal0128192 Dmel\Gβ13FΔ1-96A
Genetic reagent (D. melanogaster) UAStauGFP M. Krasnow FBtp0012358 P{UAS-tauGFP}
Genetic reagent (D. melanogaster) UASGαoGTP A. Tomlinson FBal0183487 Dmel\GαoGTP.UAS
Genetic reagent (D. melanogaster) locoΔ13 C. Klämbt FBal0096758 Dmel\locoΔ13
Genetic reagent (D. melanogaster) moodyΔ17 R. Bainton FBab0044985 Df(1)moody-Δ17
Genetic reagent (D. melanogaster) moody-RNAi R. Bainton FBtp0022779 P{UAS-moody.dsRNA}
Genetic reagent (D. melanogaster) UASnucmCherry T. Schwabe P{UAS-nuc.Cherry}
Genetic reagent (D. melanogaster) UASGFPEB1 Bloomington Drosophila Stock CenterD.Brunner BDSC:35512 Dmel\P{UAS-EB1-GFP}3
Genetic reagent (D. melanogaster) UASGFPNod Bloomington Drosophila Stock Center FBtp0014112 P{UASp-nod-GFP}
Genetic reagent (D. melanogaster) UASGFPRho Bloomington Drosophila Stock Center BDSC:9528FBal0189974 Dmel\Rho1GFP
Genetic reagent (D. melanogaster) UASRab4RFP Bloomington Drosophila Stock Center BDSC:8505FBtp0018526 9,562
Genetic reagent (D. melanogaster) UASactinGFP Bloomington Drosophila Stock Center BDSC:9562FBtp0001557 P{ActGFP}
Genetic reagent (D. melanogaster) Rho72R Bloomington Drosophila Stock Center FBal0061660 Dmel\Rho172R
Genetic reagent (D. melanogaster) Rho1B Bloomington Drosophila Stock Center BDSC:9477FBal0176027 Dmel\Rho11B
Genetic reagent (D. melanogaster) MLCK02860 Bloomington Drosophila Stock Center BDSC:11089FBal0159721 Dmel\Strn-Mlckc02860
Genetic reagent (D. melanogaster) MLCKC234 Bloomington Drosophila Stock Center BDSC:16314FBal0159722 Dmel\Strn-MlckC234
Genetic reagent (D. melanogaster) tubGAL80ts Bloomington Drosophila Stock Center BDSC:7019FBst0007019 Dmel\P{tubP-GAL80ts}20
Genetic reagent (D. melanogaster) PkaC1KK10896 VDRC VDRC:109758 FBgn0037103
Genetic reagent (D. melanogaster) Rho1KK108182 VDRC VDRC:v109420FBal0259627 Dmel\Rho1KK108182
Genetic reagent (D. melanogaster) TauGD8682 VDRC VDRC:v25023FBal0210735 Dmel\tauGD8682
Antibody Anti-PkaC1 (rabbit polyclonal) Lane ME; Genes Dev. 1993 ABS571, RRID:AB_2568479RRID:AB_2568479 IF(1:400)
Antibody Anti-PkaC1 (mouse monoclonal) Abcam ab15051, RRID:AB_2269474 IF(1:500)
Antibody Anti-REPO (mouse monoclonal) Developmental Studies Hybridoma Bank Cat#: 17-9987-42; RRID:AB_2043823 IF(1:10)
Antibody Anti-GFP (mouse monoclonal) Molecular Probes A-11120 IF(1:100)
Antibody Anti-Mega (mouse monoclonal) R. Schuh IF(1:100)
Antibody Anti-dContactin (guinea pig polyclonal) M. Bhat IF(1:100)
Antibody Anti-RFP (rabbit polyclonal) US Biological IF(1:100)
Antibody Anti-Moody b (rabbit polyclonal) Bainton et al., 2005; Schwabe et al., 2005 IF(1:500)
Software, algorithm Fiji NIH ImageJ
Software, algorithm Imaris 4.0 Bitplane
Chemical compound, drug Texas red-coupled dextran, 10 kDa Molecular Probes D1828 10 mg/ml