TABLE 2.
Level of G1 arrest conferred by mutations in STE3DAF cells and degree of supersensitivity conferred by mutations in wild-type cells
| STE4 allele | Plasmid | Mutation(s) present | % Unbudded cellsa | Halo size (mm)b |
|---|---|---|---|---|
| STE4 | YCpSTE4 | 60 (56, 63) | 39.0 | |
| STE4SD1 | YCpSD1 | R162G, C182R, I195V | 92 (91, 93) | 44.0 |
| STE4SD2 | YCpSD2 | Q17L, Q21R, M283V | 70 (68, 72) | 42.5 |
| STE4SD3 | YCpSD3 | L132I, N155D, D270G | 66 (66, 66) | 44.0 |
| STE4SD13 | YCpSD13 | Q17L, Q21R, R162G | 71 (67, 75) | 40.5 |
| STE4 | YCpLSTE4 | 51 (47, 55) | 35.5 | |
| STE4SD4 | YCpLSD4 | R162G | 43 (43, 44) | 36.0 |
| STE4SD5 | YCpLSD5 | C182R | 50 (52, 48) | 43.0 |
| STE4SD7 | YCpLSD7 | Q17L | 51 (50, 52) | 37.0 |
| STE4SD8 | YCpLSD8 | Q21R | 49 (46, 52) | 35.5 |
| STE4SD9 | YCpLSD9 | R162G, I195V | 62 (65, 59) | 35.0 |
| STE4SD10 | YCpLSD10 | Q17L, R162G | 65 (61, 69) | 36.0 |
| STE4SD11 | YCpLSD11 | Q21R, R162G | 61 (63, 59) | 36.5 |
| STE4SD12 | YCpLSD12 | Q17L, Q21R | 59 (62, 56) | 37.0 |
| STE4SD13 | YCpLSD13 | Q17L, Q21R, R162G | 71 (69, 72) | 36.5 |
| STE4SD14 | YCpLSD14 | Q17L, Q21R, R162G, I195V | 62 (60, 64) | 37.5 |
a
MATa STE3DAF strains (AC17-2B) containing the indicated plasmids were treated with 0.1 μM α-factor for 3 h, and the percentage of unbudded cells was determined. The experiment was performed with two independent transformants, and the average of the two determinations is presented, followed by the values for each experiment in parentheses.
b
Halo assays were performed on a single transformant each of a MATa strain (AC17-7B) containing the indicated plasmids using 5 μl of 1 mM α-factor. Boldfacing indicates supersensitive responses.