Table 2.

The comparison of fermentation incubation indexes. Significant differences between the means were determined by Tukey’s test. Differences were considered significant at P < 0.05

Items	LB	LC	LT	HB	HC	HT
pH	7.57 ± 0.08	7.49 ± 0.03	7.61 ± 0.10	7.59 ± 0.02	7.55 ± 0.05	7.48 ± 0.05
S2−, μg/g	15.06 ± 0.94a	14.83 ± 1.12a	12.50 ± 0.50b	12.32 ± 0.98b	12.99 ± 1.59ab	9.84 ± 1.44c
SO42−, mg/g	256.94 ± 29.93	268.52 ± 61.26	221.30 ± 79.04	257.87 ± 85.95	287.96 ± 46.39	243.98 ± 70.84
Acetate, mmol/L	31.53 ± 4.01	30.19 ± 0.96	32.81 ± 3.25	32.45 ± 1.85	34.63 ± 2.23	34.15 ± 2.70
Propionate, mmol/L	15.51 ± 1.54	15.34 ± 3.40	15.21 ± 1.35	16.00 ± 1.00	16.58 ± 0.91	15.59 ± 0.74
Butyrate, mmol/L	8.33 ± 0.54c	8.16 ± 0.56c	9.10 ± 0.73bc	10.81 ± 1.44a	10.67 ± 1.22a	10.17 ± 0.60ab
Total VFAs, mmol/L	55.37 ± 6.00	53.69 ± 3.18	57.11 ± 5.29	59.26 ± 2.94	61.88 ± 1.98	59.91 ± 3.70
Methionine, μg/mL	246.21 ± 18.83c	245.88 ± 15.01c	280.39 ± 5.78ab	257.70 ± 19.30	258.92 ± 12.08	299.12 ± 3.68a

Data are presented as means with their standard errors

Means within a row with different superscript letters differ (P < 0.05)

LB Lohmann intestinal content broth added nothing, LCs Lohmann intestinal content broth added miRNA control, LT Lohmann intestinal content broth added gga-miR-222a, HB Hy-line intestinal content broth added nothing, HC Hy-line intestinal content broth added miRNA control, HT Hy-line intestinal content broth added gga-miR-222a