TABLE 1.
Cosmids and plasmids used for transformation or hybridization experiments
| Plasmid | Vector | Insert | Reference |
|---|---|---|---|
| B3 | pANsCos1 | S. macrospora genomic DNA | 42 |
| p27.7 | pANsCos1 | Deletion derivative of cosmid clone B3 containing the complete acl1 ORF | This work |
| p41.1 | pBCHygro | 3-kb SalI restriction fragment of cosmid clone B3 containing the N-terminal part of the acl1 ORF | This work |
| p49.4 | pANsCos1 | Deletion derivative of cosmid clone B3 containing none of the acl1 ORF | This work |
| p52.9 | pBCHygro | 1.8-kb EcoRV restriction fragment of cosmid clone B3 containing C-terminal parts of the acl1 ORF | This work |
| p58.1 | pBluescriptII/KS(+) | 4.8-kb EcoRV-HindIII fragment of cosmid clone B3 containing the complete acl1 ORF; p58.1 was used for construction of plasmids p59.3, p61.2, and p94.1 | This work |
| p59.3 | pBCHygro | 3.5-kb ApaI restriction fragment of cosmid clone B3 containing the complete acl1 ORF cloned into the ApaI and SmaI sites of pBCHygro | This work |
| p61.2 | pBCHygro | 3.5-kb ApaI restriction fragment of cosmid clone B3 containing the complete acl1 ORF cloned into the ApaI site of pBCHygro | This work |
| p85.1 | p59.3 | Nucleotide exchange from T to A at the EcoRV restriction site within the acl1 ORF | This work |
| p94.1 | pBCHygro | 3.5-kb ApaI restriction fragment of cosmid clone B3 containing the complete acl1 ORF with the HA epitope introduced into the PstI site of the ORF | This work |